Molecular Image Detail

Image

Caption

Generation of the Mbd5^tm1.1Gxu and Mbd5^tm1.2Gxu alleles. (A) The strategy. Numbered black boxes represent the coding exons and open boxes represent the 5' untranslated region (UTR). The LoxP and Frt sites are shown as black and gray triangles, respectively. The floxed region contains exon 1, whch encodes the majority of the MBD domain. The probes used in the Southern blot analysis to correctly identify targeted ES cells are indicated by the horizontal bars. The restriction sites used to digest ES genomic DNA were BamHI (B) and EcoRV (E). The PCR primers used for genotyping are indicated with arrows P1, P2 and P3.

This image is from Du Y, PLoS One 2012;7(10):e47358, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:192212

Associated
Alleles

Symbol	Name
Mbd5^tm1.1Gxu	methyl-CpG binding domain protein 5; targeted mutation 1.1, Guo-Liang Xu
Mbd5^tm1.2Gxu	methyl-CpG binding domain protein 5; targeted mutation 1.2, Guo-Liang Xu

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