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| Caption | Epcam gene inactivation scheme for the RST412 and RST413 clones to generate the EpcamGt(RST412)Byg and EpcamGt(RST413)Byg alleles. The inactivating exonic cassette within the pGT1TMPF gene-trap vector (top) contains an ATG-less open reading frame (ORF) of beta-galactosidase fused to neomycin phosphotransferase (beta-GEO, cyan), followed by an internal ribosome entry site (black) for placental alkaline phosphatase (PLAP) ORF (red); simian virus 40 transcription termination/polyadenylation signal (SVpA, gray); an intronic sequence from Engrailed2 (EN2) (red line) provides a strong splicing acceptor site (red arrowhead). The insertion brings the promoterless bicistronic b-GEO-PLAP-SVpA cassette in the third intron of wild-type (WT) Epcam (middle). Cassette insertion leads to a null (KO) Epcam, by leading to the splicing of the Epcam exon 3 to the beta-GEO cassette (bottom). Yellow boxes, Epcam untranslated exon sequences; green boxes, Epcam translated exons; arrows, PCR primer positions. | ||||||
| Copyright | This image is from Guerra E, PLoS One 2012;7(11):e49302, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:195002 | ||||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/19/2024 MGI 6.24 |
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