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| Caption | Generation of the Nkx2-2tm4.1(cre/EGFP)Suss allele. Nkx2-2LCA is the RMCE acceptor allele integrated into 4H9 ES cells (Arnes et al., 2012). RMCE lox sites are indicated by colored arrows. The targeting vector includes an Frt-flanked hygro selection cassette. The cre/EGFP gene was recombineered in-frame at the Nkx2-2 ATG codon and deleted most of coding exon 1. Ex I' signifies the truncated region of coding exon 1 and Ex II refers to the coding exon 2. The cre/EGFP cassette replaces the LCA allele using RMCE. P1 and P2 = 5' PCR primers that verify proper integration of the hygromycin cassette. P3 and P4 = 3' primers that distinguish the wild-type allele and the newly integrated cre/EGFP allele with the 2722 lox site. Flpe recombinase removed the Frt-flanked selection cassette. P5 and P6 = primer set used to identify the cre/EGFP allele. | ||||
| Copyright | This image is from Balderes DA, Genesis 2013 Dec;51(12):844-51, and is displayed with the permission of Wiley-Blackwell, who owns the Copyright. J:204532 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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