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| Caption | Targeting strategy for the generation of the Rhotm2.1Kpal allele. Rhotm2.1Kpal mice were generated by using a targeting vector containing a 2.11-kb 5' short homology arm (SA) and a 5.36 kb long homology arm (LA). A loxP-flanked Neo cassette was upstream of exon 2. The target 284-bp sequence region included a G to A transversion at codon 150 in exon 2 (star). Codon 150 in exon 2 was changed to AAG by homologous recombination, and the flipase recognition target loxP-flanked Neo cassette was inserted before exon 2. The Neo cassette was removed by Cre-loxP recombination. Whole-locus sequencing confirmed the knock-in (KI). This mouse retained a 44-bp loxP sequence in the intron between exons 1 and 2. | ||||
| Copyright | This image is from Zhang N, J Clin Invest 2013 Jan 2;123(1):121-37 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:194158 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/19/2024 MGI 6.24 |
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