Molecular Image Detail

Image

Caption

Targeting of the ROSA26 locus. A, top to bottom: pBigT, plasmid containing a loxP-flanked cassette with a PGK-neo selection marker and tpA transcriptional stop sequence, followed by the EYFP or ECFP sequence; pROSA26PA, containing genomic ROSA26 sequences for homologous recombination, and a diphtheria toxin gene (PGK-DTA) for negative selection in ES cells; wild type ROSA26 locus, with the location of the probe indicated; the structure of the targeted allele (Gt(ROSA)26Sor^tm1(EYFP)Cos or Gt(ROSA)26Sor^tm2(ECFP)Cos ); the structure of the cre-recombined allele Gt(ROSA)26Sor^{tm1.1(EYFP)Cos}. Solid arrowheads indicate loxP sites.

This image is from Srinivas S, BMC Dev Biol 2001;1(1):4, an open-access article, licensee BioMed Central Ltd. J:80963

Associated
Alleles

Symbol	Name
Gt(ROSA)26Sor^{tm1.1(EYFP)Cos}	gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Frank Costantini
Gt(ROSA)26Sor^tm1(EYFP)Cos	gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Frank Costantini
Gt(ROSA)26Sor^tm2(ECFP)Cos	gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Frank Costantini

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