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Caption Strategy for generation of the Pi4katm1.1Pdc (floxed allele) and Pi4katm1.2Pdc (Knockout allele) alleles. We elected to flank exons 48-52 of Pi4ka with loxP sites, as they encode for a large portion of the highly conserved kinase domain. Neo, neomycin resistance; TK, thymidine kinase. Kpn1 and NsiI denote restriction sites. Green, location of hybridization of Southern blot probe; purple, PCR amplicon to verify presence of 5' loxP site (expected amplicon size is 337 bp in floxed allele and 317 bp in wild-type allele).
Copyright This image is from Nakatsu F, J Cell Biol 2012 Dec 10;199(6):1003-16, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:195235
Associated
Alleles
Symbol Name
Pi4katm1.1Pdc phosphatidylinositol 4-kinase alpha; targeted mutation 1.1, Pietro De Camilli
Pi4katm1.2Pdc phosphatidylinositol 4-kinase alpha; targeted mutation 1.2, Pietro De Camilli

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/29/2024
MGI 6.24
The Jackson Laboratory