Molecular Image Detail

Image

Caption

Targeting strategy and generation of Mob1a^tm1.1Asuz and Mob1a^tm1.2Asuz alleles. (A) Construction of the Mob1a targeting vector and mutant allelles. Top: The 4.7 kb and 2/7 kb HindIII fragments diagnostic of the wild-type (WT) and targeted Mob1a alleles, respectively, are shown. WT allele: A portion of the mouse Mob1a WT locus showing the KpnI (K) HindIII (H), and BamHI (B) sites. Targeting vector: Targeting vector with the hygromycin resistance gene (Hygr) in reverse orientation to Mob1a transcription. Exon 2 was flanked by two loxP sequences (arrowheads), with a third loxP sequence flanking Hygr. Targeted allele: The mutated Mob1a^ta allele containing three loxP sequences and the Hygr gene. Flox allele: Mob1a^tm1.1Asuz (Mob1a^flox) allele produced after Cre-mediated Hygr deletion. Delta allele: Mob1a^tm1.2Asuz (Mob1a^delta) allele produced after Cre-mediated deletion. Bottom: The 9.1 kb, 6.5 kb, and 4.7 kb KpnI fragments diagnostic of the WT Mob1a allele, the Mob1a^flox allele and Mob1a^delta allele, respectively, are shown. The 5'-flanking probe (Probe A) and a 3' long arm probe (Probe B) are also indicated.

This image is from Nishio M, J Clin Invest 2012 Dec 3;122(12):4505-18 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:193981

Associated
Alleles

Symbol	Name
Mob1a^tm1.1Asuz	MOB kinase activator 1A; targeted mutation 1.1, Akira Suzuki
Mob1a^tm1.2Asuz	MOB kinase activator 1A; targeted mutation 1.2, Akira Suzuki

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