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Caption Generation of the Chpftm1.1Hwat and Chpftm1.2Hwat alleles. A, Genomic structure of Chpf gene, the targeting vector, and the genome with homologous recombination and that whose fragments were excised by flippase and Cre systems. The genomic structure of Chpf gene is depicted on the top line (a). Chpf targeting vector (b) was constructed by flanking exon 1 with loxP sites, and flanking a NeoR cassette with FRT sites. ES cell clones with homologous recombination of the vector segment (c) were obtained by positive selection, and used for generation of chimera mice, followed by germ line transmission. The FRT-flanked Neo cassette was subsequently deleted from the recombinant allele by Flp-mediated recombination to generate the Chpftm1.1Hwat allele (d). Then, a genomic fragment containing exon 1, flanked by loxP sites, was excised from the recombinant allele by crossing with Tg(CAG-cre)13Miya conditional mice to generate the Chpftm1.2Hwat allele (e).
Copyright This image is from Ogawa H, PLoS One 2012;7(8):e43806, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:191659
Associated
Alleles
Symbol Name
Chpftm1.1Hwat chondroitin polymerizing factor; targeted mutation 1.1, Hideto Watanabe
Chpftm1.2Hwat chondroitin polymerizing factor; targeted mutation 1.2, Hideto Watanabe

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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory