Molecular Image Detail

Image

Caption

Generation of the floxed Psmc4^tm1.1Ryot and knock-out Psmc4^tm1.2Ryot alleles. A, schematic diagram of the Psmc4 (Rpt3) floxed construct to generate the Psmc4^tm1.1Ryot allele. Cre removes the region of the Psmc4 gene that encodes exons 7-10 (black boxes), which are flanked by loxP (triangles) and generates the Psmc4^tm1.2Ryot allele. The neomycin resistance gene (white box) between FRT (closed half-circles) was already removed by crossing Tg(EEF1A1-flp)66Mim (FLP66) mice with Psmc4^tm1.1Ryot floxed mice. F1, Psmc4 (Rpt3) fl-for primer; R1, Psmc4 (Rpt3) fl-rev primer; R2, Psmc4 (Rpt3) del-rev primer; A, Aatl; AL, ApaLI; H, HindIII.

This image is from Tashiro Y, J Biol Chem 2012 Dec 14;287(51):42984-94 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:193770

Associated
Alleles

Symbol	Name
Psmc4^tm1.1Ryot	proteasome (prosome, macropain) 26S subunit, ATPase, 4; targeted mutation 1.1, Ryosuke Takahashi
Psmc4^tm1.2Ryot	proteasome (prosome, macropain) 26S subunit, ATPase, 4; targeted mutation 1.2, Ryosuke Takahashi

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 11/12/2024 MGI 6.24