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| Caption | Gene targeting for the Pax1tm2.1Tlu (Pax1EGFP:Pax1-) construct. Genomic organization of Pax1 represented in a line diagram. Yellow boxes represent the exons (numbered accordingly); black solid lines, intronic sequences; white boxes, untranslated region (UTR); pink box, IRES; orange box, F2A peptide; red triangles, FRT sites; green boxes, EGFP; blue boxes, Neo cassette; purple solid lines, external Southern blotting probes; green solid lines, internal Southern blotting probes; blue arrows, primer pairs. E; EcoRI and St, Stu1. (b) Pax1tm2.1Tlu targeting construct illustrating the insertion of the F2A-EGFP-FRT-PGK-gb2-Neo-FRT cassette (2.43 kb) immediately before the translational stop codon of Pax1. The expected fragment size from EcoRI digestion for the mutant allele, as detected by hybridization probes 1 and 2, is 5.14 kb (dotted line). Bottom: line drawing depicting the allele after FLPe-mediated excision of Neo selection cassette in mice. | ||||
| Copyright | This image is from Sivakamasundari V, Genesis 2013 Jun;51(6):420-9, and is displayed with the permission of Wiley-Blackwell, who owns the Copyright. J:198603 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 12/10/2024 MGI 6.24 |
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