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| Caption | Generation of the Pgrtm4.1Lyd (PRflox) and Pgrtm4.2Lyd (PRd) alleles. (a) Schematic shows the gene-targeting strategy and relevant mouse crosses to generate mice carrying the PRflox and PRd alleles. The targeting vector contains 7.9 kb of PR homologous sequence in which the 5' and 3' arms of homology are 2.9 and 5 kb, respectively. Within the 5' arm, exon 2 is engineered to be flanked by loxP sites (gray triangles) in which the5' loxP site also contains a novel Bam HI site (B*). The FRTNEOFRT cassette (NEO) is inserted in intron 2 in which the neomycin resistance gene (neo) is flanked by direct FRT repeats (black triangles). The HSV-TK gene is attached to the 3' end of the targeting vector to allow negative selection. Homologous recombination with the PR allele in ES cells results in the generation of the PRflox(neo) targeted allele in which exon 2 is floxed, and the FRTNEOFRT cassette is inserted in intron 2. Mice carrying the targeted allele were crossed with Gt(ROSA)26Sortm1(FLP1)Dym/Gt(ROSA)26Sortm2Dym (FLP deleter) mice to remove the FRTNEOFRT cassette to generate mice harboring the Pgrtm4.1Lyd (PRflox) allele in which exon 2 is floxed but phenotypically normal. The PRflox mouse was crossed with the Tg(Zp3-cre)3Mrt/0 (Zp3 Cre) mouse to excise exon 2. Following cre mediated excision, a single loxP and FRT site remain in the intron of the Pgrtm4.2Lyd (PRd) allele. Bam HI, BglII, and Xho I are denoted by B, b, and X, respectively. The positions of the PCR primers used to identify mice carrying the wild-type (WT) and PRd allele are indicated by p, q, and r. | ||||||
| Copyright | This image is from Fernandez-Valdivia R, Genesis 2010 Feb;48(2):106-13, and is displayed with the permission of Wiley-Blackwell, who owns the Copyright. J:158406 | ||||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/19/2024 MGI 6.24 |
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