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| Caption | Generation of the Gabrg2tm1.1(Gabrg2)Wwis (gamma2F77/177) gene switch mouse version (gamma2 knockdown allele). A, Targeting strategy. A full length Gabrg2 cDNA was placed into the 5'UTR of exon 1 of the Gabrg2 gene. The Gabrg2 cDNA encodes the 177 version of the gamma2 subunit. The 5' loxP contains a BglII restriction site. For the initial targeting a frt-flanked neomycin resistance gene (neo) was placed after the Gabrg2 cDNA. The neo cassette contains a second loxP site at the end, the 3' loxP site. The 5' frt site contains another BglII site. Black bars indicate the positions of Probe A and B. Arrows are PCR primer positions. Black triangle, loxP sites; black diamonds, frt sites; B, BglII site; S, SalI site; Sp, SpeI site; TGA, STOP codon; UTR, untranslated region; HA, hemagglutinin epitope; polyA, polyadenylation signal. | ||||
| Copyright | This image is from Frola E, PLoS One 2013;8(2):e56311, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:199326 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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