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| Caption | Generation of the Mir21atm1.1Zqy allele. (A) Diagram for Mir21a (miR21) exon 1-floxed targeting construct and location of primers used for genotyping of targeted locus and FLP, Cre-mediated excised targeted locus. The exon 1 of Mir21a and 3.2 kb, 5kb genomic fragments flanking exon 1 (left and right arm) were PCR-amplified and subcloned into a targeting vector such that exon 1 of Mir21a was followed by a neo cassette, and exon 1 was flanked by two loxP sites, neo was flanked by two FRT sites. This targeting construct was used to generate Mir21a flox/flox(neo) mice, which were crossed with FLP mice to excise the neo and generate the Mir21atm1.1Zqy allele (FLP-excised targeted locus). Mice can then be conditionally targeted by crossing with Cre-expressing mice to generate the Cre-excised targeted locus. | ||||
| Copyright | This image is from Zhang XY, PLoS One 2013;8(3):e59002, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:199907 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 08/02/2024 MGI 6.24 |
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