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| Caption | Generation of the Scube3tm1(KOMP)Vlcg allele. (A) Domain organization of Scube3 encoded by the Scube3-001 splice variant. This protein consists of a signal peptide sequence (S), nine EGF-like repeat domains, a spacer region containing three cysteine-rich repeats, a CUB domain and C-terminal sequence; (B) The murine Scube3-001 splice variant incorporates 6699 base pairs and contains 22 exons. Exon 1 encodes the signal peptide seuence, exons 2-10 encode the nine EGF-like domains, exons 11-18 encode the spacer region and cysteine residues in proximity to the CUB domain, exons 19 and 20 encode the CUB domain itself and exons 21-22 encode the amino acid sequence present at the C-terminal region of the protein, downstream of the CUB domain; (C) The Scube3-004 splice variant incorporates 4103 base pairs and contains 18 exons. The splice variants are aligned to each other, meaning that exon 1 of Scube-004 corresponds to exon 3 of Scube3-001 and so forth. This is interrupted by exon skipping at exon 16 in Scube-001, such that intron 13-14 of Scube-004 corresponds to intron 15-16, exon 16 and intron 16-17 of Scube3-001 (a total region of 714 base pairs). Exon 18 of Scube3-004, which corresponds in part, to exon 21 of Scube-001 is also modified by an alternative 5' splice site, incorporating 1684 'extra' base pairs from the adjacent intronic sequence (intron 21-22 of Scube-001). Red boxes represent exons, white boxes represent untranslated regions of exon 1 and 22; (D) The targeting vector containsa ZEN-Ub1 cassette consisting of a lacZ-p(A) reporter and hUbCpro-neo-p(A) selectable marker flanked by loxP sites, which replaces the whole Scube3 coding region following homologous recombination in ES cells (A-D are not to scale). | ||||||
| Copyright | This image is from Xavier GM, PLoS One 2013;8(1):e55274, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:195784 | ||||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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