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| Caption | Generation of the Dact3tm1.1Yegc allele. A, shown is a schematic diagram of the wild-type (WT) allele, targeting vector, Dact3 allele containing the floxed neo cassette (Dapper3neo-flox), and Dact3tm1.1Yegc (Dapper3KO) allele. Boxes with numbers represent exons. Arrows mark the position of the PCR primers used for genotyping. The location of the 5' outside and 3' outside probes for Southern blot analysis are indicated. Solid triangles denote loxP sites; open triangles denote frt sites. Neo and TK are positive and negative selection genes, respectively. Besides restriction site PvuI, two additional sites for Southern blot were introduced in the targeting vector: a BamHI site between 5' loxP site and Exon 2 and an XbaI site between 3' loxP site and Exon 4. The 5' homologous arm of the targeting vector is 5.7 kb, and the 3' arm is 4.5 kb. Dapper3neo-flox allele is created through homologous recombination between WT allele and targeting vector. Dapper3 KO allele, Dact3tm1.1Yegc, was converted from Dapper3neo-flox allele by crossing to transgenic cre mice. | ||||
| Copyright | This image is from Xue H, J Biol Chem 2013 May 24;288(21):15006-14 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:199623 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 12/10/2024 MGI 6.24 |
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