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Caption Generation of the Prkcitm1.1Rfar (PKCiota/lambda delta) allele. Targeting of the Prkci (PKCi) locus in mice. Schematic representation of the targeting strategy is shown. Restriction maps indicating the relevant restriction sites of the wild-type (wt) and mutant locus are shown (B, BamHI; Bg, BglII; E, EcoRI; EV, EcoRV; H, HindIII; K, Kpnl). The black box represents the second exon of the Prkci gene which was used for all targeted modifications. The white boxes below the mutant alleles indicate the 5' HindIII-EcoRI RFLP probe (5' probe) and the 3' KpnI-EcoRI RFLP probe (3' probe) used for later southern blot analysis. B) Conditional knockout - Prkci (PKCi) wild-type (wt) locus, the targeting construct (containing a single LoxP site 5' and a floxed neo cassette 3' of the 2nd exon) and the mutant Prkci locus (PKCi floxed allele) after homologous recombination and subsequent deletion via Cre recombinase are shown.
Copyright This image is from Seidl S, PLoS One 2013;8(5):e62756, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:202124
Associated
Alleles
Symbol Name
Prkcitm1.1Rfar protein kinase C, iota; targeted mutation 1.1, Robert V Farese Sr

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last database update
10/29/2024
MGI 6.24
The Jackson Laboratory