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| Caption | Generation of the Prkcitm1.1Rfar (PKCiota/lambda delta) allele. Targeting of the Prkci (PKCi) locus in mice. Schematic representation of the targeting strategy is shown. Restriction maps indicating the relevant restriction sites of the wild-type (wt) and mutant locus are shown (B, BamHI; Bg, BglII; E, EcoRI; EV, EcoRV; H, HindIII; K, Kpnl). The black box represents the second exon of the Prkci gene which was used for all targeted modifications. The white boxes below the mutant alleles indicate the 5' HindIII-EcoRI RFLP probe (5' probe) and the 3' KpnI-EcoRI RFLP probe (3' probe) used for later southern blot analysis. B) Conditional knockout - Prkci (PKCi) wild-type (wt) locus, the targeting construct (containing a single LoxP site 5' and a floxed neo cassette 3' of the 2nd exon) and the mutant Prkci locus (PKCi floxed allele) after homologous recombination and subsequent deletion via Cre recombinase are shown. | ||||
| Copyright | This image is from Seidl S, PLoS One 2013;8(5):e62756, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:202124 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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