Molecular Image Detail

Image

Caption

Generation of a chromosome 13 deletion, Del(13)1Mgld. A megadeletion (MD) mouse model for the rat Mcs1a locus reveals Nr2f1 as the Mcs1a target gene. A) Schematic representation of the rat Mcs1a locus and mouse orthologous locus that was deleted using mutagenic insertion and chromosome engineering resource (MICER) vector-assisted targeting. The horizontal bar, genetic markers and chromosomal base pair positions in purple represent the location of the Mcs1a critical interval on rat chromosome 2 (UCSC Genome Browser, rn4). The marker and base pair position in light purple delineate the location of the distal end of the Mcs1a critical interval at the time of design of the MICER project. The MD on mouse chromosome 13 is depicted as a red horizontal bar. The coordinates (in bp) are from the 2007 version of the mouse genome (UCSC Genome Browser, mm9). At the indicated base pair positions, a compatible pair of MICER vectors was placed, such that upon Cre-recombinase-driven excision in the embryonic stem-cell stage, a functional Hypoxanthine-guanine phosphoribosyltransferase (HPRT) gene was formed, which allowed for selection of a correctly targeted ES cell clone. Construct MHPP256h04 contained Agouti (A), HPRT exons 3', loxP (grey triangle) and a Puromycin resistance gene (puro); Construct MHPN5k06 contained a Neomycin resistance gene (neo), loxP (grey triangle), HPRT exons 1 and 2, and Tyrosinase (TYR).

This image is from Smits BM, PLoS Genet 2013 Jun;9(6):e1003549, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:199158

Associated
Alleles

Symbol	Name
Del(13)1Mgld	deletion, Chr 13, Michael N Gould

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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