Molecular Image Detail

Image

Caption

Targeted conditional deletion of FAT1 transmembrane domain. (A) Strategy used to generate the conditional allele. 1 - Top: genomic organization of the Fat1 locus around the targeted area. 2 - Targeted Fat1 locus, in which exons 24 and 25 (the latter containing the transmembrane domain) were flanked by LoxP sites creating the Fat1^tm1Fhel (Fat1^Fln) allele. The locus also contains a pgk-neo selection cassette, itself flanked by LoxP sites (yellow triangles) and by FRT sites (bluetriangles, for later removal of the pgk-neo cassette only). An external probe (red bar) was used to identify recombinant ES clones by Southern blotting. The sizes of the Nco1 restriction fragments are indicated. 3 -Neo cassette removal is permitted by FRT-mediated excision of the neo cassette, which is flanked with both FRT (blue) and LoxP (yellow) sites. This generates the Fat1^tm1.1Fhel (Fat1^Flox) allele, in which exons 24-25 are flanked on the 5' side with one LoxP site, and onthe 3' side with one leftover FRT site, followed with a LoxP site. 4 - CRE-Recombined Fat1 locus: Genome organisation of the targeted Fat1 locus after cre-mediated excision of the entire fragment comprised between loxP sites, including exons 24-25 and the neo cassette. This new recombined allele is referred to as Fat1^tm1.2Fhel (Fat1^deltaTM) allele. Primers indicated (1 to 7) are the ones used for genotyping by PCR. ES screening primers are givenin the method section.

This image is from Caruso N, PLoS Genet 2013 Jun;9(6):e1003550, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:199157

Associated
Alleles

Symbol	Name
Fat1^tm1.1Fhel	FAT atypical cadherin 1; targeted mutation 1.1, Francoise Helmbacher
Fat1^tm1.2Fhel	FAT atypical cadherin 1; targeted mutation 1.2, Francoise Helmbacher
Fat1^tm1Fhel	FAT atypical cadherin 1; targeted mutation 1, Francoise Helmbacher

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