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| Caption | Schematic of the gene-trapped Fat1Gt(KST249)Byg hypomorphic allele. In the Fat1Gt(KST249)Byg (Fat1LacZ) insertion allele (ES line name Fat1KST249), multiple copies of a secretory-trap vector were inserted in tandem downstream of the first exon of the Fat1 gene. As a result, the main product of the Fat1 locus is a fusion protein of 291 kDa, including the first exon of Fat1 (the first 8 cadherin domains), in frame with the exogenous transmembrane and beta-geo fusion protein. (A, top) Schematic representation of the gene trap vector and its insertion point in the mouse Fat1 locus. With its splice acceptor site, the depicted cassette behaves as an exon. Following (in blue), is an element encoding a transmembrane domain, and a beta-geo fusion reporter (in frame fusion of the beta-galactosidase and the neomycin resistance gene). The ES selection procedure ensures that this reporter cassette is in frame with the preceding exon (exon 1 of mouse FAT1). The resulting FAT1-beta-gal fusion is a transmembrane protein. This protein is recognized by antibodies raised against an epitope of FAT1 mapping in exon 1 (Fat1-1869), but not by an antibody raised against a downstream epitope in the extracellular domain (Fat1-23882). | ||||
| Copyright | This image is from Caruso N, PLoS Genet 2013 Jun;9(6):e1003550, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:199157 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 12/17/2024 MGI 6.24 |
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