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| Caption | Panel A. Targeting Strategy. Nfat5tm1.1Itl (TonEBP-floxed) mice were generated by "inGenious targeting Laboratory, Inc." (2200 Smithtown Avenue, Ronkonkoma, NY 11779) using standard gene-targeting techniques. The targeting construct was designed such that the short homology arm extends about 2.45 kb 3' to exon 4, whereas the long homology arm extends about 5.25 kb 5' to exon 4. A single loxP-site, containing an engineered Stul-site for Southern Blot analysis, was inserted 166 bp upstream of exon 4 and a loxP/FRT-flanked Neo cassette was inserted 202 bp downstream of exon 4. Thus the target region is 561 bp long and includes exon 4. The figure is not exactly drawn to scale. BA1 hybrid embryonic stem cells were electroporated with 10 ug of linearized targeting vector and selected with G418 antibiotic. | ||||
| Copyright | This image is from Wiig H, J Clin Invest 2013 Jul 1;123(7):2803-15 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:201429 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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