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| Caption | (B) Schematic representation of the Sox2tm1.1Vlcg (Sox2COIN) and Sox2tm1.2Vlcg (Sox2INV) alleles. The COIN module intron is inserted after the 30th nucleotide of Sox2's coding region (i.e. coordinate 34549367 on Chromosome 3) splitting the single exon of Sox2 into two exons and also dividing the CpG island. The COIN module is comprised of an optimized gene trap-like element composed of the 3' splice region of the rabbit beta globin gene (HBB_RABIT), followed by eGFP (lacking an initiating ATG) and the polyadenylation region from HBB_RABIT, all placed in the antisense strand. The COIN module has been flanked with Lox71 and Lox66 sites in a mirror image orientation, thereby enabling inversion by Cre. For BHR and targeting, a FRT-flanked neo cassette has been incorporated into the COIN intron. After targeting, neo is removed to give rise to the Sox2COIN allele. The COIN module is antisense to Sox2, and hence predicted not to interfere with expression of Sox2. However, after inversion of the COIN module to the sense strand, transcription terminates around the polyadenylation region of the COIN module, and as a result expression of Sox2 is replaced by eGFP in the Sox2INV allele. | ||||||
| Copyright | This image is from Mandalos N, PLoS One 2012;7(9):e45768, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:191980 | ||||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 10/22/2024 MGI 6.24 |
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