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| Caption | Generation of the Ubr4tm1.1Nkt allele. (A) Targeting strategy of Ubr4 (p600) locus. The exon encoding the initiating methionine codon of Ubr4 allele was replaced with a Neo cassette by homologous recombination in ES cell lines. The ES cell lines were microinjected into mouse blastocysts to generate chimeric mice. These chimeras were bred to obtain offspring that are heterozygous for Neo allele. Heterozygous mice were then crossed with EIIa-Cre transgenic mouse to delete the loxP flanked region, yielding the mutant allele. The locations of PCR amplicons used for genotyping are as indicated. Restriction enzyme digestion of genomic DNA with BamHI and SpeI produces the 2.5 and 4.3 kbp fragments from WT and Neo alleles, respectively, that hybridize with the probe for Southern blotting. The regions for PCR genotypings are indicated. | ||||
| Copyright | This image is from Nakaya T, PLoS One 2013;8(6):e66269, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:203460 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/19/2024 MGI 6.24 |
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