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| Caption | The strategy for generating the Cxcr2tm1Rmra (Cxcr2-flox) allele. (a) Schematic diagram of the Cxcr2 wild-type genomic locus demonstrating a classic two-exon gene structure that is indicated by two blank boxes. In order to screen for the targeted Cxcr2tm1Rmra allele, both 5' and 3' Southern blot probes (filled boxes) were designed in the genomic regions inside of two homologous arms (HA). Restriction enzymes are in the abbreviated form: Ec, EcoRV, and Ba, BamHI. (b) A Cxcr2fl targeting vector was constructed by recombineering. The targeting region includes a 2-kb 5' HA, a LoxP site that flanks Cxcr2 exon2, a Frt-PGKNeo-Frt cassette that is downstream of Cxcr2 exon2 flanked by another LoxP site and a 3-kb 3' HA. (c) Schematic demonstration of the targeted Cxcr2tm1Rmra (Cxcr2fl) allele. In Southern blot screening, an 8.8 kb-BamHI targeted band (5' probe) and an 11kb-EcoRV targeted band (3' probe) are expected. (d) Schematic diagram depicting the conditional deletion of the Cxcr2 gene through Cre mediated recombination. | ||||
| Copyright | This image is from Liu L, Genesis 2013 Aug;51(8):587-95, and is displayed with the permission of Wiley-Blackwell, who owns the Copyright. J:204482 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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