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| Caption | Generation of the Gbx1tm1Mrh allele. Inactivation of the mouse Gbx1 gene by homologous recombination in embryonic stem (ES) cells. (A) The upper drawing shows the restriction map of the wild-type locus, boxes and lines corresponding to exons and introns, respectively. The homeodomain sequence is in red. In the targeting vector (below), two Gbx1 genomic fragments (between the dashed lines) flank a lacZ reporter gene and the neomycin resistance gene (grey box), transcribed in the same orientation (thin arrow) as Gbx1. In the recombined locus (lower drawing), 313 bp of Gbx1 exon 2 (including the homeodomain) are replaced by the lacZ neo sequence. The location of the 3' probe used for Southern blot analysis of ES cells is indicated in blue, and the PCR primers used to distinguish wild-type and recombined alleles for genotyping of animals (F1, R1, LacZ R2) are also indicated. | ||||
| Copyright | This image is from Meziane H, PeerJ 2013;1():e142, an open-access article distributed under the terms of the Creative Commons Attribution License. J:201154 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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