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| Caption | Generation of the Mesdtm2Bch (floxed) and Mesdtm2.1Bch (LoxP) alleles. (A) Comparison of the Mesdc2 Wild-type, Floxed, and LoxP Mesdc2 alleles and targeting vector. The WT allele (top map) has three exons (1-3) that are designated by grey rectangles. The first exon encodes the signal peptide that directs the MESDC2 protein into the ER as well as the N-terminal helical region essential for maturation of LRPs. The 3' untranslated portion of the third exon is indicated by light grey. In the Mesdc2 targeting vector, we introduced the Neomycin resistance gene (Neo) (white rectangle) flanked by Flp-recombinase sites (green triangles) into the Mesdc2 first intron. In addition, we introduced three LoxP sites (red triangles) flanking the first and second exons and the Neo gene. The targeting construct was introduced into C57BL/6 embryonic stem cells (Ozgene),and cell lines heterozygous for the Floxed allele (middle map) were identified by Southern analysis using the 5P, enP, and P3 probes, whose positions are indicated by black rectangles. Based on Southern analysis using these probes, we determined that only the first two LoxP sites were incorporated into ES cells; the recombination sites are indicated by dashed lines. Expression of Cre-recombinase will trigger recombination between LoxP sites to generate the LoxP allele (bottom map). | ||||||
| Copyright | This image is from Taibi AV, PLoS One 2013;8(10):e75782, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:209024 | ||||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 06/12/2024 MGI 6.13 |
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