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| Caption | Generation of the Hspb1tm1.1Jdn allele. A, Schematic showing the size of Spe I fragments detected by Probe Q in Southern blot. Spe I sites separated by 9.8 kb flank the endogenous Hspb1 locus containing exons 1-3 (hashed boxes). Translational start (ATG) and stop codons are indicated. The introduction of a Flp-recombinase (FRT)-flanked neomycin cassette (NEO) and LoxP sites at 5' and 3' positions of exons 1-3 by homologous recombination introduces a new Spe I site, and Probe Q now recognises a 3 kb fragment. The Cre-recombinase-excised locus produced by crossing floxed Hspb1 mice with CMV-CRE deleter strain is indicated in which the endogenous Spe I sites are separated by 6.5 kb. B, Southern blot with Probe Q for four founder mice showing WT, knockout and recombined alleles of the expected size. M denotes DNA ladder (lengths in kb). | ||||
| Copyright | This image is from Crowe J, PLoS One 2013;8(10):e77383, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:209117 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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