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| Caption | Generation of the Galntl5tm1Nari allele. (A, Top) Normal allele showing the first three of the nine exons comprising mouse Galntl5. (Middle) Targeting construct with the neomycin resistance gene (Neo) and the thymidine kinase gene (TK) as selectable markers. Homologous recombination occurred with 6.0-kbp and 3.4-kbp regions encompassing the first and third exons, respectively. (Bottom) The targeted allele, where the second exon, including the translational start site, has been replaced with a Neo cassette. Numbered boxes indicate exons, thick horizontal lines represent homologous DNA regions, and bars and gray arrows indicate the positions of the 5' probe and the PCR primers (6605A, 5970S, NeoLeft25, and AvrHind2-2), respectively. Restriction enzyme sites are indicated by A (AvrII), H (HindIII), P (PmlI), and S (SpeI). | ||||
| Copyright | This image is from Takasaki N, Proc Natl Acad Sci U S A 2014 Jan 21;111(3):1120-5. Copyright 2014 National Academy of Sciences, U.S.A. J:206466 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 12/10/2024 MGI 6.24 |
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