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| Caption | Generation of the Esr1tm1.1Ics allele. The targeting vector was constructed as follows: An 809-bp fragment encompassing Esr1 exon 7 and part of intron 6 and 7 was PCR-amplified on RP24-73H17 C57BL/6J BAC in two steps to allow the introduction of the point mutation of the base G into C (giving C451A) in exon 7. This fragment was subcloned into an ICS proprietary vector that contains a floxed neomycin resistance cassette (NeoR). The 5' homology arm (4.3 kb) and 3' homology arm (3.5 kb) were then sequentially subcloned into the same vector. The linearized construct was electroporated in C57BL/6NTac mouse embryonic stem (BD10 ES) cells. | ||||
| Copyright | This image is from Adlanmerini M, Proc Natl Acad Sci U S A 2014 Jan 14;111(2):E283-90. Copyright 2014 National Academy of Sciences, U.S.A. J:206373 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/19/2024 MGI 6.24 |
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