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| Caption | Generation of the Prdm6tm1.1Jrld (flox) and Prdm6tm1.2Jrld (del) alleles. (B) Targeting strategy for homologous recombination at the Prdm6 locus. The region containing exon 3 of the Prdm6 wild-type (wt) locus, the targeting vector and the distinct recombinant alleles are shown. The homology arms for recombination aredrawn as strong lines and the probe region used for Southernblot analysis is indicated. LoxP sites are represented by triangles, the neomycin selection cassette by NEO and the FRT sites by closed circles. Homologous recombination in ES cells produced the recombinant (rec) locus containing the LoxP-flanked exon 3 region and the FRT-flanked NEO cassette. ES cells carrying the rec locus were then transferred into the germ line of mice. Crossing mice with the rec locus with flp deleter mice resulted in deletion of the NEO cassette and generated the flox allele, Prdm6tm1.1Jrld. Crossing flox mice with Cre deleter mice resulted in the deletion of exon 3, thus generating the del allele, Prdm6tm1.2Jrld. | ||||||
| Copyright | This image is from Gewies A, PLoS One 2013;8(11):e81833, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:209773 | ||||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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