Molecular Image Detail

Image

Caption

Generation of the Cpeb4^tm1.1Yshu allele (KO CPEB4). (A) Schematic illustration of the targeting strategy. The Cpeb4 gene consists of 10 exons (numbered boxes) and spans a region of approximately 60-kb (WT, wild-type). The targeting vector containing the flip recombinase target (FRT)-flanked neomycin-resistance gene (Neo) and loxP-flanked exon 2 cassette was inserted into the Cpeb4 gene by homologous recombination. The correctly targeted locus was examined by Southern blotting using DNA digested with BamHI (B1) or BglII (B2) and probed with a 5'- end or 3'-end probe, respectively. The mouse line carrying the floxed allele of CPEB4 was then crossed with a transgenic male mouse expressing Cre recombinase in the sperm (protamine-Cre) to produce the knockout (KO) allele, Cpeb4^tm1.1Yshu.

This image is from Tsai LY, PLoS One 2013;8(12):e84978, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:209837

Associated
Alleles

Symbol	Name
Cpeb4^tm1.1Yshu	cytoplasmic polyadenylation element binding protein 4; targeted mutation 1.1, Yi-Shuian Huang

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