Molecular Image Detail

Image

Caption

Generation of the Patz1^tm1.2Welm allele. A) Schematic map of Patz1 (MAZR, 641 aa) showing the N-terminal BTB domain and the 7 zinc fingers at the C-terminus. B) Schematic map of the targeting construct (top panel), the endogenous Patz1 (Mazr) gene locus (middle panel), the targeted Patz1 gene locus after homologous recombination (Mazr^+/Fneo-, upper bottom panel), and the targeted locus after the neomycin cassette deletion (Mazr^+/F; middle bottom panel) which generates the Patz1^tm1.2Welm allele. Cre-mediated recombination can be used to delete exon 1 (Mazr^+/delta; lower bottom panel). All EcoRI (E), XhoI (X) and EcoRV (EV) restriction sites are shown. Open arrowheads (1, 2, and 3) indicate the location of the PCR primers used to detect deletion of exon 1. The horizontal thick black line (in the top and bottom panel) indicates the region of homology between the targeting construct and the endogenous locus. The thick bar (in the middle part) represents the 5' probe used for Southern-blotting. Horizontal bars with numbers (indicating the size in kb) show the expected genomic fragments after digestion with the appropriate restriction enzyme (EcoRI for the 5' targeted region).

This image is from Abramova A, PLoS One 2013;8(10):e77677, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:209168

Associated
Alleles

Symbol	Name
Patz1^tm1.2Welm	POZ (BTB) and AT hook containing zinc finger 1; targeted mutation 1.2, Wilfried Ellmeier

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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