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| Caption | (B) Construction strategy of the Del(4Cyp2j13-Cyp2j5)1Brs deletion. The wild-type (WT) 5' BAC and WT 3' BAC were modified using 5' and 3' targeting vectors, respectively, through homologous recombination in E. coli. The resultant MT5' BAC and MT3' BAC lack sequences between the recombination arms (HR) and have acquired selectable markers (neomycin resistance, thymidine kinase sensitivity, and blasticidin resistance) and integrase sites (attB1 and attP1 of TP901, attB2 and attP2 of R4). The fused BAC (FS BAC) was generated through site-specific recombination between attB3 and attP3 sites carried by the MT5' BAC and MT3' BAC, respectively, and mediated by TP901 integrase. Ampr, Ampicillin resistance; Bsrr, blasticidin resistance; TK, herpes simplex thymidine kinase; neor, kanamycin/geneticin resistance. | ||||
| Copyright | This image is from Zhou GL, PLoS Genet 2013 Nov;9(11):e1003950, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:223128 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 09/24/2024 MGI 6.24 |
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