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| Caption | Generation of the Pde4diptm1Mhda allele. Strategy for generating DUF1220 domain knockout mice and verification of genotype. a The DUF1220 domain is encoded by parts of exons 31 and 32 (green boxes). Primers (purple boxes) designed to upstream ("left arm") and downstream ("right arm") regions were used to amplify PDE4DIP without DUF1220. The two arms were ligated together. An HpaI site was introduced into the right arm, and this was used to insert PGK/neo cassette for later selection of positive transformants by neomycin resistance. This vector was transfected into mouse ES cells and incorporated into the mouse genome by homologous recombination. | ||||
| Copyright | This image is from Keeney JG, Mamm Genome 2014 Oct 12;(): and is displayed with the permission of Springer Science + Business Media, Inc., New York who owns the copyright. J:216968 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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