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| Caption | Generation of the Utf1tm1Okud allele. (A) Schematic representation of the UTF1-targeting strategy. Top: Restriction enzyme map of the mouse UTF1 locus. Middle: Structure of the UTF1-targeting vector carrying neomycin (Neo)-resistance and TK genes for positive and negative selection, respectively. Bottom: Knock-in allele of the UTF1-targeting vector. I and II in black boxes indicate exons 1 and 2 of the UTF1 gene, respectively. Red line corresponds to 7.6 kb BglII genomic fragment from wild-type UTF1 locus, while magenta and blue lines correspond to 4.7 and 3.4 kb BglII/ Nco1 genomic fragments from the targeted UTF1 locus detected with 5' and 3' probes, respectively. The 3.4 kb BglII/Nco1 genomic fragment can be also detectable by the Neo Probe. | ||||
| Copyright | This image is from Nishimoto M, PLoS One 2013;8(7):e68119, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:204409 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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