| Image |
|
||||
| Caption | Generation of the Myh9tm1.1Dash allele. A) Targeting strategy for R702C knock-in mutation of the murine Myh9 gene. The targeting vector pMulti-ND1.0_Myh9neo, the wild-type (Myh9WT) Myh9 allele and the targeted allele before (Myh9neo) and after Cre mediated excitation of the loxP franked neo cassette (Myh9mt) are schematically represented. The targeting vector also contains the Diphtheria toxin fragment A (DTA) gene outside the flanking homologies. Black boxes in the genomic structures represent exon sequences; the asterisk on exon 16 denotes the R702C mutation; the underlined N (NdeI) denotes the extra diagnostic restriction site created by silent mutation. The expected restriction fragments of the genotyping PCR products are indicated with their relative size accompanied with solid lines. The black box above the WT gene represents the 5' probe used for Southern blot analysis. The open arrowheads under the gene represent the primers used for long-PCR genotyping. | ||||
| Copyright | This image is from Suzuki N, PLoS One 2013;8(8):e71187, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:204923 | ||||
|
Associated Alleles |
|
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
||
|
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support. |
last database update 11/19/2024 MGI 6.24 |
|
|
|
||
Analysis Tools