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| Caption | Generation of the Del(16Hspa13-App)3Yah and Dp(16Hspa13-App)2Yah mice. (A) The 9.2 Mb targeted region defined by the Hspa13 and App genes as shown from a capture derived from the UCSC genome browser (http://genome.ucsc.edu/). Black headband: chromosomes bands; blue headband: contigs; red rectangle boxes: protein coding genes. (B) The targeting vectors containing a loxP site (green arrow), a selectable antibiotic resistance gene (puro or neo), and a part of the Hprt gene (3' or 5' Hprt, red arrows) were integrated successively in the Hspa13 locus (Hspa13tm1Yah) and in the App gene (Apptm1Yah). The start and end positions of the genomic homologous regions present in the 5' and 3'-Hprt vectors are from mouse genome assembly GRm38. The Hspa13tm1Yah allele was checked by Southern blot analysis. The digestion of DNA with SacI (S) restriction enzyme and hybridisation with external probes A and B gives additional fragments of 19.7 kb and 14.3 kb, respectively, for the Hspa13tm1Yah allele compared to the wild-type (wt) allele of 17.2 kb. The hybridisation of the same DNA digest with the Gap1 probe gives two additional fragments of 19.7 and 14.3 kb for the Hspa13tm1Yah. (C) The integration of the 3'Hprt vector was confirmed in the same manner by digestion with the PsiI (P) enzyme and hybridisation with probes C (wt, 11.5 kb; Apptm1Yah allele, 10.9 kb), D (wt, 11.5 kb; Apptm1Yah allele, 9.9 kb), and Gap2 (wt, 11.5 kb; Apptm1Yah allele, 10.9 and 9.9 kb). (D) After Cre-mediated recombination, the deletion (Ms3Yah allele) and duplication (Ts3Yah allele) were identified by NdeI (N) digestion, and a probe was made on the ampicillin gene (Ampi) present in both targeting vectors that recognises recombinant clones with the Ms3Yah (23.2 kb) and Ts3Yah (18.2 kb) alleles from nonrecombinant ones that still carry the Hspa13tm1Yah (26 kb) and Apptm1Yah (15.4 kb) alleles. Puro: puromycin, neo: neomycin. | ||||||
| Copyright | This image is from Brault V, PLoS Genet 2015 Mar;11(3):e1005062, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:223518 | ||||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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