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| Caption | Generation of the Mybtm2.1Epr (c-mybF) and Mybtm2.2Epr (c-mybdelta) alleles. (a) Strategy for flanking exon 9A of the murine Myb (c-myb) locus with loxP sites. The partial structure of the Myb locus containing exons 6-14 is depicted as well as the targeting vector, which contains two selectable markers (arrows): thymidine kinase (tk) and neomycin resistant gene (neo). LoxP sites are shown as arrowheads. MybF[tkneo] is the product of homologous recombination that is used as a substrate for subsequent recombination by Cre recombinase. The position of the probe used to determine homologous recombination and for additional genomic Southern blotting studies of the targeted p89 c-myb allele structure is shown. Deletion of the tk-neo markers generates the conditional c-mybF allele in ES cells, whereas deletion of the loxP-flanked exon 9A (open box) generates the desired systemic, p89c-myb null mutant allele (c-mybdelta). B, BamHI; E, EcoRI; S, SalI; X, XhoI. | ||||||
| Copyright | This image is from Baker SJ, Genesis 2010 May;48(5):309-16, and is displayed with the permission of Wiley-Blackwell, who owns the Copyright. J:160189 | ||||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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