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| Caption | Generation of the P2ry14tm1.1Shbg allele. Mutant mice were generated at Takeda Ltd. The coding sequence was partly replaced by an Encephalomyocarditis virus-internal ribosome entry site (EMCV-IRES)/Beta-galactosidase cassette followed by a loxP-floxed neomycin selection cassette. We removed neomycin cassette by breeding with EIIa-Cre mice, and successful removal was verified by PCR. For qPCR, a primer pair matching to exon 1 and 2 was designed. Genotyping of mice was performed with the primers indicated: forward (forw), reverse (rev), and Beta-galactosidase sense (Beta-gal s). | ||||
| Copyright | This image is from Meister J, J Biol Chem 2014 Aug 22;289(34):23353-66 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:223427 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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