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| Caption | Generation of the Tjp1tm1.1Whun allele. (A) Targeting strategy using the Cre/loxP system. Schematic of the mouse genomic locus for Tjp1 (ZO-1 gene) is shown. The targeting vector introduces loxP-FRT-flanked Neo and F3-loxP-flanked Puro resistance cassettes 5' and 3', respectively, of Tjp1 exon 4. After Flp recombination, the conditional allele, Tjp1tm1.1Whun, contains a loxP-flanked exon 4 with residual 5' FRT and 3' F3 sites. Cre-recombination can be used to delete the floxed exon 4, yielding the constitutive KO allele. Genotyping PCR primers to identify wild-type, Tjp1-floxed heterozygous and homozygous animals are indicated with orange bars. The drawing is not to scale. | ||||
| Copyright | This image is from Itoh M, PLoS One 2014;9(9):e106621, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:226210 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 12/10/2024 MGI 6.24 |
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