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| Caption | Generation of the Adra2atm1Qiwa allele. A, schematic illustration of gene knock-in at the ADRA2A locus. ADRA2A is an intronless gene. The HA sequence was added to the 5'-end of the coding region. The two triangles flanking the neo cassette represent the loxP sites. The two numbered boldface lines below the gene structure represent the probes used in Southern analyses, and the two paired arrowheads represent the primers used in PCR analysis. HR, homologous recombination in embryonic stem (ES) cells. F1 heterozygotes bearing the targeted ADRA2A locus were crossed with Cre mice to excise the neo cassette. The Cre mouse line used in these studies has stable and sufficient expression of the Cre recombinase enzyme in all cells to mediate deletion of any loxP-flanked locus, such as the neo cassette present in our initial targeting vector, in all cells of the mouse. Cre, Cre recombinase-mediated recombination. E, EcoRI restriction enzyme digestion site; B, BamHI digestion site. | ||||
| Copyright | This image is from Lu R, J Biol Chem 2009 May 8;284(19):13233-43 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:149752 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/19/2024 MGI 6.24 |
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