Molecular Image Detail

Image

Caption

Generation of the Dmxl2^{tm1a(EUCOMM)Wtsi} and Dmxl2^{tm1c(EUCOMM)Wtsi} alleles. The EUCOMM Dmxl2 gene trap allele with conditional potential and the Dmxl2 allele after the action of Cre recombinase. (A) The Dmxl2 gene trap allele (tm1a allele) contains an IRES:LacZ trapping cassette with an acceptor splice site (En2 SA) and a floxed neoR cassette. LoxP sites flank the critical exon 7 and the neoR cassette. FRT sites flank the IRES:LacZ and neoR cassettes (adapted from www.knockoutmouse.org/about/eucomm). The IRES:LacZ trapping cassette and the floxed neoR cassette have been deleted by the FLIP recombinase to generate Dmxl2^lox/lox (Dmxl2^{tm1c(EUCOMM)Wtsi} allele) mice. The critical exon 7 was then deleted by the Nestin cre-recombinase to generate both Dmxl2^lox/wt and nes-Cre;Dmxl2^-/wt. Dashed lines indicate possible RNA splice isoforms. Single-headed orange or blue arrows represent the positions of the PCR primers used to genotype mice.

This image is from Tata B, PLoS Biol 2014 Sep;12(9):e1001952, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:217623

Associated
Alleles

Symbol	Name
Dmxl2^{tm1a(EUCOMM)Wtsi}	Dmx-like 2; targeted mutation 1a, Wellcome Trust Sanger Institute
Dmxl2^{tm1a(EUCOMM)Wtsi}	Dmx-like 2; targeted mutation 1a, Wellcome Trust Sanger Institute
Dmxl2^{tm1c(EUCOMM)Wtsi}	Dmx-like 2; targeted mutation 1c, Wellcome Trust Sanger Institute

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