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| Caption | Generation of the Guca1atm1.1Hunt (Guca1aCOD3 knock-in) allele with E155G mutation in Guca1a (GCAP1). (a) A vector targeting the endogenous Guca1a locus was constructed to include an A-to-G transversion at nucleotide position 19 in exon 4 of Guca1a (red circle), as well as a loxP-flanked (blue arrow heads) neomycin resistance gene within intron 3. Following Cre-mediated excision of the Neo selectable marker, the resulting locus contained the A- to- G change in exon 4 plus a residual 34 bp loxP sequence in intron 3. This was used to distinguish between mutant and native alleles by PCR - the position of the primers used is indicated by the small blue arrows on the Cre-deleted Guca1a locus. | ||||
| Copyright | This image is from Buch PK, PLoS One 2011;6(3):e18089, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:171667 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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