Molecular Image Detail

Image

Caption

Generation of the Snap23^tm1Paro (Snap23 ^Neo+fl), Snap23^tm1.1Paro (Snap23^delta), and Snap23^tm1.2Paro (Snap23^fl) alleles. (A)Schematic representation of the genomic structure of wild-type and targeted alleles of Snap23. Homologous recombination with the targeting construct inserts a neomycin-resistance gene (PKG-NEO) and exon 2 (E2) flanked by three loxP sites represented as triangles. HSK-tk was used for negative selection. Exon numbers with relative position, 5'- and 3'- flanking Southern blotting, and a partial restriction map are indicated. Restriction sites: C, ClaI; E, EcoRI; K, KpnI; S, SpeI. Female Snap23^Neo+fl/wt heterozygous mouse (Snap23^tm1Paro) harboring the targeted allele (F1) was bred with male EIIa-Cre transgenic mice. Offspring harboring mosaic alleles (F2) through partial and/or total excision of loxP-flanked sequences were generated. EIIa-Cre+ F2 mosaic male mice were further bred with C57BL/6 female mice to separate the Snap23^fl/wt, Snap23^Neo+fl/wt, and Snap23^Delta/wt alleles as shown (F3)

This image is from Suh YH, PLoS One 2011;6(3):e18444, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:171444

Associated
Alleles

Symbol	Name
Snap23^tm1.1Paro	synaptosomal-associated protein 23; targeted mutation 1.1, Paul A Roche
Snap23^tm1.2Paro	synaptosomal-associated protein 23; targeted mutation 1.2, Paul A Roche
Snap23^tm1Paro	synaptosomal-associated protein 23; targeted mutation 1, Paul A Roche

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