About   Help   FAQ
Molecular Image Detail
Image
Caption Schematic of the Gt(ROSA)26Sortm1(NOTCH3)Sat, Gt(ROSA)26Sortm2(NOTCH3*C455R)Sat and Gt(ROSA)26Sortm3(NOTCH3*R1031C)Sat constructs. Schematic representation of the targeting vectors used to generate conditional knock-in mice expressing human Notch 3 wild-type (hNOTCH3 WT; Gt(ROSA)26Sortm1(NOTCH3)Sat), or the C455R (Gt(ROSA)26Sortm2(NOTCH3*C455R)Sat) or R1031C (Gt(ROSA)26Sortm3(NOTCH3*R1031C)Sat) mutant receptors. Each construct contains genomic sequences allowing for homologous recombination into the ROSA locus; an adenovirus splice acceptor (SA); a PGK-neo-tpA "stop" cassette flanked by loxP sites (black triangles) allowing for Cre-mediated excision; the transgene; and the bovine growth hormone polyadenylation sequence (bpA). The NOTCH 3 WT and mutant transgenes are followed by an internal ribosomal entry site (IRES) and nuclear EGFP (nEGFP).
Copyright This image is from Arboleda-Velasquez JF, Proc Natl Acad Sci U S A 2011 May 24;108(21):E128-35. Copyright 2011 National Academy of Sciences, U.S.A. J:171887
Associated
Alleles
Symbol Name
Gt(ROSA)26Sortm1(NOTCH3)Sat gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Spyros Artavanis-Tsakonas
Gt(ROSA)26Sortm2(NOTCH3*C455R)Sat gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Spyros Artavanis-Tsakonas
Gt(ROSA)26Sortm3(NOTCH3*R1031C)Sat gene trap ROSA 26, Philippe Soriano; targeted mutation 3, Spyros Artavanis-Tsakonas
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory