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| Caption | Generation of the Pak2tm1Rjak allele. a) Schematic representation of the PAK2 wild-type allele, targeting vector, and recombinant allele. Exons 7-10 and selected restriction enzyme sites are indicated. The targeting vector was designed to replace Asp-212 with Asn-212 to create a caspase cleavage-deficient D212N mutant and to insert a SV-Neo gene cassette with flanking loxP sites into intron 7. The targeting vector contains a 2081-bp 5'-homologous region that contains exon 7 and a 5568-bp 3'-homologous region that contains exons 8-10 for homologous recombination. The 5'-homologous region with the D212N mutation in exon 7 was generated by inserting a PCR product that contains the D212N mutation and flanking XbaI/XhoI sites into the XbaI/XhoI sites of the 4523G9 vector. The 3'-homologous region was generated by inserting a BamHI/XbaI fragment into the BglII/NheI sites of the 4523G9 vector. The positive selection marker neomycin resistance gene (SV-Neo) is indicated within the recombination region of the targeting vector and the recombinant allele, and the negative selection markers herpes simplex virus thymidine kinase (HSV-TK) and dipthera toxin (DTA) are indicated outside the recombination region of the targeting vector. | ||||
| Copyright | This image is from Marlin JW, Mamm Genome 2011 Jun;22(5-6):306-17 and is displayed with the permission of Springer Science + Business Media, Inc., New York who owns the copyright. J:171914 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 10/22/2024 MGI 6.24 |
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