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| Caption | Generation of the Bicd2tm1Hgrd (Bicd2f) and Bicd2tm1.1Hgrd (Bicd2-) alleles. A) The mouse BICD2 locus and gene targeting constructs. To generate an inducible Bicd2 knockout allele, we introduced a neomycin-resistance gene surrounded by loxP sequences into intron 1, and a puromycin selection marker, surrounded by loxP sequences and followed by Beta-galactosidase (lacZ) reporter gene fused to a nuclear localization signal (NLS), downstream of the mouse Bicd2 gene. The top line represents Bicd2, with exons indicated by solid boxes (white boxes, 5' and 3' UTRs; black boxes, coding regions). Exon 1 contains the start codon (ATG) and exon 9 contains the stop codon (asterisk). The positions of Southern-blot probes 1 and 2 (horizontal lines) and PCR primers a, b, c and d (arrows) are indicated. Selected restriction enzyme sites are shown (BamHI and XbaI). The targeting constructs are shown below Bicd2. Homology with the Bicd2 gene is indicated, as are the lengths of the homologous regions. The loxP sites are represented by arrows. NEO, neomycin resistance cassette; PURO, puromycin-resistance cassette; TK, thymidine kinase gene; HA-NLS-lacZ, HA- and NLS-tagged lacZ cassette, containing an engineered splice acceptor site (3' splice) and polyadenylation signal (not indicated). The doubly targeted BICD2 allele, Bicd2f (targeted), is shown below the targeting constructs. Cre-mediated recombination at the outermost loxP sites of the Bicd2f allele removes most of the Bicd2 sequences and generates the Bicd2- allele, which is represented by the bottom line. The splice acceptor site at the 5' end of the reporter lacZ cassette can be spliced onto Bicd2 exon 2 sequences, generating a hybrid Bicd2-lacZ transcript. | ||||||
| Copyright | This image is from Jaarsma D, Nat Commun 2014;5():3411, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:210236 | ||||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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