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| Caption | Generation of the Ate1tm2.1Avar allele. (A) The 5' end of the previously produced unconditional Ate1- allele, in which the Ate1 exons 1b through 3 were replaced by a cassette encoding a promoter-lacking, NLS-containing LacZ (NLS-beta-gal) (it was expressed from the endogenous P(Ate1) promoter) and the Neo selection marker expressed from the phosphoglycerate kinase PPGK promoter (green rectangles). (B) A diagram of the 5' end of wild-type mouse Ate1, indicating approximate locations of exons 1a through 5. (C) The ~22.5 kb targeting construct containing a ~6 kb long-arm region of Ate1 homology (shown as a shaded rectangle on the left); a single loxP site (red triangle) upstream of Ate1 exon 2, a "floxed"-hygromycin-resistance (hph) cassette, expressed from the P(PGK) promoter (blue arrow between two red triangles) downstream of Ate1 exon 4; a ~2 kb short-arm region of homology (an inclined shaded rectangle), and the HSV thymidine kinase (tk) negative-selection cassette expressed from the P(HSV) promoter (yellow arrow). Wavy line indicates an abutting sequence of the pBR322 plasmid DNA. (D) The tri-lox Ate1 allele obtained after a correctly targeted double crossover event. (E) In the notations here and elsewhere in the paper, "flox-on" indicates a configuration depicted in this panel (the functionally active Ate1flox allele), whereas "flox-off" indicates a configuration depicted in panel F (the null Ate1- allele). The functionally active, "flox-on" (Ate1flox; Ate1tm2.1Avar) allele, obtained by the removal of the hph cassette, using the in vivo expression of Cre-recombinase driven by the P(EIIA) promoter, which is active only in pre-implantation blastocysts. (F) The null "flox-off" (Ate1-) allele obtained by conditional cre-mediated recombination, in this case the inducible expression of CreER recombinase from the P(Cagg) promoter and post-translationally induced by tamoxifen (TM) treatment. | ||||
| Copyright | This image is from Brower CS, PLoS One 2009;4(11):e7757, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:155421 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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