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| Caption | Generation of the Gt(ROSA)26Sortm3(rtTA,tetO-cre/ERT)Nat allele. The rtTA coding region is preceded by a splice acceptor so that it can be spliced onto the 5' exon of the endogenous Rosa26 transcript, and it is followed by multiple transcription termination signals (stop). In the presence of doxycycline, the rtTA protein activates transcription from the seven head-to-tail Tet response elements immediately 5' of a minimal cytomegalovirus immediate early promoter (TRE), leading to expression of the CreER coding region. The resulting CreER protein translocates to the nucleus upon binding to 4-hydroxytamoxifen (4HT). For the Gt(ROSA)26Sortm3(rtTA,tetO-cre/ERT)Nat (R26rtTACreER) allele, the frt-flanked PGK-Neo selectable marker used during ES culture was removed by Flp-mediated recombination in the mouse germline. Scale bars in B and C are 1 Kb. | ||||
| Copyright | This image is from Badea TC, PLoS One 2009;4(11):e7859, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:155417 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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