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| Caption | Generation of the Pcyt2tm1.1Suja allele. A, diagram (not to scale) shows the targeting plasmid at the top, highlighting the genomic insertion cassette that confers cellular resistance to neomycin (NeoTK). Line 2 shows the structure of the wild-type (WT) allele that was replaced by recombination with the insertion cassette. Line 3 shows the gene structure after recombination in embryonic stem cells. Line 4 shows the floxed (fl) allele that resulted from a cross between mice carrying the insertion cassette and transgenic mice expressing the FLP recombinase. FLP mediated excision of the NeoTK selection cassette left only short DNA sequences within the two introns encoding two LoxP sites and a residual Frt site. These were called floxed mice and served as the WT littermate controls. Line 5 shows the conditionally deleted allele that results from cre-mediated recombination. The deleted allele lacked exon 2 and retained a single 34-bp LoxP site in the intron between exons 1 and 3. F1 is the forward PCR primer, and R1 and R2 are the two reverse PCR primers used for genotyping. | ||||
| Copyright | This image is from Leonardi R, J Biol Chem 2009 Oct 2;284(40):27077-89 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:155630 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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