Molecular Image Detail

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Caption

Generation of Tcf3^tm3Cmu (Tcfe2a^fl_E12), Tcf3^tm3.1Cmu (Tcfe2a^deltaE12), Tcf3^tm4Cmu (Tcfe2a^fl_E47), and Tcf3^tm4.1Cmu (Tcfe2a^deltaE47) alleles. Structure of the targeted Tcfe2a locus. The alternatively spliced E12 and E47 exons, respectively, were flanked with loxP sites (indicated by arrows). In addition, a tetracysteine tag (black bar) was inserted into exon 20, 3' of the coding region, to produce tagged E47 and E12 proteins. Furthermore, a neomycin (neo) resistance gene was introduced to screen for targeted ES cells. The EcoRI (E) fragments as well as the probe used for screening of the ES cells are indicated. After crossing of Tcf3^tm3Cmu (Tcfe2a^fl_E12) or Tcf3^tm4Cmu (Tcfe2a^fl_E47)-bearing mice with a germline Cre-deleter strain (Ella-Cre), the corresponding Tcf3^tm3.1Cmu (Tcfe2a^deltaE12) or Tcf3^tm4.1Cmu (Tcfe2a^deltaE47) strains were obtained.

This image is from Beck K, J Exp Med 2009 Sep 28;206(10):2271-84, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:153423

Associated
Alleles

Symbol	Name
Tcf3^tm3.1Cmu	transcription factor 3; targeted mutation 3.1, Cornelis Murre
Tcf3^tm3Cmu	transcription factor 3; targeted mutation 3, Cornelis Murre
Tcf3^tm4.1Cmu	transcription factor 3; targeted mutation 4.1, Cornelis Murre
Tcf3^tm4Cmu	transcription factor 3; targeted mutation 4, Cornelis Murre

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